Pro-tumorigenic activity of PYCR1 in gastric cancer through regulating the PI3K/AKT signaling.

ABSTRACT:

Background

The primary objective of this investigation was to assess the impact of pyrroline-5-carboxylate reductase 1 (PYCR1) on the progression of gastric cancer (GC), specifically focusing on tumor growth and metastatic potential.

Methods

Surgical specimens from patients with different stages of GC were assayed for PYCR1 expression using immunohistochemistry. PYCR1 expression was manipulated by depletion or overexpression approaches in GC cells, and these cells were applied to explore the functional roles of PYCR1. Expression of apoptosis- and metastasis-related markers was quantified through quantitative real-time PCR and Western blot.

Results

Higher PYCR1 expression was ascertained in surgical specimens from patients with GC as compared to noncancerous adjacent t

SUBMITTER: Xiao H 

PROVIDER: S-EPMC10918153 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature