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Quantifying differentiation of progenitor populations using cerebral organoid models for neurodevelopmental disorders.

ABSTRACT: Neurodevelopmental disorders are characterized by complex phenotypes that often result from concomitant dysregulation of cell proliferation, differentiation, or other crucial developmental processes. Here, we present a protocol to quantify differentiation of progenitor populations during early stages of neurogenesis in induced pluripotent stem cell (iPSC)-derived cerebral organoids. We describe steps for organoid differentiation and maturation, sample preparation, immunofluorescence, and imaging and analysis using epifluorescence microscopy. This protocol can be used to compare cerebral organoids from control and patient-derived iPSCs. For complete details on the use and execution of this protocol, please refer to Rakotomamonjy et al. (2023).¹.

SUBMITTER: Schroder AL

PROVIDER: S-EPMC10918321 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Quantifying differentiation of progenitor populations using cerebral organoid models for neurodevelopmental disorders.

Schroder Annika L AL Fairbanks-Santana Martin M Rakotomamonjy Jennifer J Guemez-Gamboa Alicia A

STAR protocols 20240229 1

Neurodevelopmental disorders are characterized by complex phenotypes that often result from concomitant dysregulation of cell proliferation, differentiation, or other crucial developmental processes. Here, we present a protocol to quantify differentiation of progenitor populations during early stages of neurogenesis in induced pluripotent stem cell (iPSC)-derived cerebral organoids. We describe steps for organoid differentiation and maturation, sample preparation, immunofluorescence, and imaging ...[more]

PMID: 38427568

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