Open Thermal Control System for Stable Polymerase Chain Reaction on a Digital Microfluidic Chip.

ABSTRACT: In this paper, a digital microfluidic thermal control system was introduced for the stable polymerase chain reaction (PCR). The system consists of a thermoelectric cooler unit, a thermal control board, and graphical-user-interface software capable of simultaneously achieving temperature control and on-chip droplet observation. A fuzzy proportional-integral-derivative (PID) method was developed for this system. The simulation analysis was performed to evaluate the temperature of different reagents within the chip. Based on the results, applying fuzzy PID control for PCR will enhance the thermal stability by 67.8% and save the time by 1195 s, demonstrating excellent dynamic response capability and thermal robustness. The experimental results are consistent with the simulation results on the

SUBMITTER: Ji J 

PROVIDER: S-EPMC10918803 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature