An improved medium for in vitro studies of female reproduction and oviposition in Schistosoma japonicum.

ABSTRACT:

Background

Schistosomiasis is a disease primarily caused by eggs laid by pathogens called schistosomes. Among the schistosome species infecting humans, Schistosoma japonicum possesses the largest fecundity; each adult female produces an average of 3500 eggs per day. The lack of proper culture conditions supporting continuous oviposition in vitro has precluded detailed investigation of mechanisms regulating sexual maturation and egg production in Schistosoma japonicum.

Methods

We optimized in vitro culture conditions by replacing reagents that are part of the classical ABC169 medium. Fast Blue BB staining and 4',6-diamidino-2-phenylindole (DAPI) labeling were applied to observe the sexual development status of the females. In vitro RNA interference (RNAi) technology was used

SUBMITTER: You Y 

PROVIDER: S-EPMC10918852 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature