Pyruvate kinase M in germ cells is essential for sperm motility and male fertility but not spermatogenesis.

ABSTRACT: Male germ cells employ specific metabolic pathways throughout their developmental stages. In a previous study, we discovered heightened expression of pyruvate kinase M (PKM), a pivotal glycolytic enzyme, in spermatogonia and spermatids. To gain deeper insights into PKM's roles in spermatogenesis, sperm function, and male fertility, we engineered a conditional-knockout mouse model ( Pkm -vKO mice) to selectively disrupt the Pkm gene within germ cells. Despite maintaining regular testicular histology and sperm morphology, the male Pkm -vKO mice were infertility, characterized by significant impairments in sperm motility and adenosine triphosphate (ATP) generation. In addition, Pkm -null spermatozoa exhibited similar deficits in protein tyrosine phosphorylation linked to capacitation, as well

SUBMITTER: Qian GQ 

PROVIDER: S-EPMC10919421 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature