Ginseng-derived type I rhamnogalacturonan polysaccharide binds to galectin-8 and antagonizes its function.

ABSTRACT:

Background

Panax ginseng Meyer polysaccharides exhibit various biological functions, like antagonizing galectin-3-mediated cell adhesion and migration. Galectin-8 (Gal-8), with its linker-joined N- and C-terminal carbohydrate recognition domains (CRDs), is also crucial to these biological processes, and thus plays a role in various pathological disorders. Yet the effect of ginseng-derived polysaccharides in modulating Gal-8 function has remained unclear.

Methods

P. ginseng-derived pectin was chromatographically isolated and enzymatically digested to obtain a series of polysaccharides. Biolayer Interferometry (BLI) quantified their binding affinity to Gal-8, and their inhibitory effects on Gal-8 was assessed by hemagglutination, cell migration and T-cell

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC10920006 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature