Reduction of renal interstitial fibrosis by targeting Tie2 in vascular endothelial cells.

ABSTRACT:

Background

Tie2, a functional angiopoietin receptor, is expressed in vascular endothelial cells and plays an important role in angiogenesis and vascular stability. This study aimed to evaluate the effects of an agonistic Tie2 signal on renal interstitial fibrosis (RIF) and elucidate the underlying mechanisms.

Methods

We established an in vivo mouse model of folic acid-induced nephropathy (FAN) and an in vitro model of lipopolysaccharide-stimulated endothelial cell injury, then an agonistic Tie2 monoclonal antibody (Tie2 mAb) was used to intervent these processes. The degree of tubulointerstitial lesions and related molecular mechanisms were determined by histological assessment, immunohistochemistry, western blotting, and qPCR.

Results

Tie2 mAb attenuated RIF and redu

SUBMITTER: Jiang L 

PROVIDER: S-EPMC10920200 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature