A SWI/SNF-dependent transcriptional regulation mediated by POU2AF2/C11orf53 at enhancer.

ABSTRACT: Recent studies have identified a previously uncharacterized protein C11orf53 (now named POU2AF2/OCA-T1), which functions as a robust co-activator of POU2F3, the master transcription factor which is critical for both normal and neoplastic tuft cell identity and viability. Here, we demonstrate that POU2AF2 dictates opposing transcriptional regulation at distal enhance elements. Loss of POU2AF2 leads to an inhibition of active enhancer nearby genes, such as tuft cell identity genes, and a derepression of Polycomb-dependent poised enhancer nearby genes, which are critical for cell viability and differentiation. Mechanistically, depletion of POU2AF2 results in a global redistribution of the chromatin occupancy of the SWI/SNF complex, leading to a significant 3D genome structure change and a sub

SUBMITTER: Szczepanski A 

PROVIDER: S-EPMC10920751 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature