Extracellular vesicles released by microglia and macrophages carry endocannabinoids which foster oligodendrocyte differentiation.

ABSTRACT:

Introduction

Microglia and macrophages can influence the evolution of myelin lesions through the production of extracellular vesicles (EVs). While microglial EVs promote in vitro differentiation of oligodendrocyte precursor cells (OPCs), whether EVs derived from macrophages aid or limit OPC maturation is unknown.

Methods

Immunofluorescence analysis for the myelin protein MBP was employed to evaluate the impact of EVs from primary rat macrophages on cultured OPC differentiation. Raman spectroscopy and liquid chromatography-mass spectrometry was used to define the promyelinating lipid components of myelin EVs obtained in vitro and isolated from human plasma.

Results and discussion

Here we show that macrophage-derived EVs do not promote OPC differentiation, and th

SUBMITTER: Lombardi M 

PROVIDER: S-EPMC10921360 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature