Aquaporin-8 promotes human dermal fibroblasts to counteract hydrogen peroxide-induced oxidative damage: A novel target for management of skin aging.

ABSTRACT: The skin is subjected to various external factors that contribute to aging including oxidative stress from hydrogen peroxide (H₂O₂). This study investigated the distribution of aquaporin-8 (AQP8), a protein that transports H₂O₂ across biological membranes, in skin cells, and its effects in mitigating H₂O₂-induced oxidative damage. Human dermal fibroblasts were treated with increasing concentrations of H₂O₂ to evaluate oxidative damage. Cell viability, reactive oxygen species (ROS) generation, and the expression of specific genes associated with skin aging (IL-10, FPR2, COL1A1, KRT19, and Aggrecan) were evaluated and AQP8 expression was assessed via quantitative polymerase chain reaction and western blot

SUBMITTER: Liu SH 

PROVIDER: S-EPMC10921499 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature