Organelle resolved proteomics uncovers PLA2R1 as a novel cell surface marker required for chordoma growth.

ABSTRACT: Chordomas are clinically aggressive tumors with a high rate of disease progression despite maximal therapy. Given the limited therapeutic options available, there remains an urgent need for the development of novel therapies to improve clinical outcomes. Cell surface proteins are attractive therapeutic targets yet are challenging to profile with common methods. Four chordoma cell lines were analyzed by quantitative proteomics using a differential ultracentrifugation organellar fractionation approach. A subtractive proteomics strategy was applied to select proteins that are plasma membrane enriched. Systematic data integration prioritized PLA2R1 (secretory phospholipase A2 receptor-PLA2R1) as a chordoma-enriched surface protein. The expression profile of PLA2R1 was validated across chordoma

SUBMITTER: Khan S 

PROVIDER: S-EPMC10921702 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature