RNA-seq analysis of chlorogenic acid intervention in duck embryo fibroblasts infected with duck plague virus.

ABSTRACT:

Introduction

Chlorogenic acid, the primary active component in Chinese medicines like honeysuckle, exhibits anti-inflammatory and antiviral effects. It has been demonstrated that chlorogenic acid effectively prevents and treats Duck enteritis virus (DEV) infection. This study aims to further elucidate the mechanism by which chlorogenic acid prevents DEV infection.

Methods

Duck embryo fibroblast (DEF) cells were pre-treated with chlorogenic acid before being infected with DEV. Cell samples were collected at different time points for transcriptomic sequencing, while qPCR was used to detect the proliferation of DEV. Additionally, 30-day-old ducks were treated with chlorogenic acid, and their lymphoid organs were harvested for histopathological sections to observe pathological d

SUBMITTER: Yang Y 

PROVIDER: S-EPMC10921813 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature