Circulating extracellular choline acetyltransferase regulates inflammation.

ABSTRACT:

Background

Choline acetyltransferase (ChAT) is required for the biosynthesis of acetylcholine, the molecular mediator that inhibits cytokine production in the cholinergic anti-inflammatory pathway of the vagus nerve inflammatory reflex. Abundant work has established the biology of cytoplasmic ChAT in neurons, but much less is known about the potential presence and function of ChAT in the extracellular milieu.

Objectives

We evaluated the hypothesis that extracellular ChAT activity responds to inflammation and serves to inhibit cytokine release and attenuate inflammation.

Methods

After developing novel methods for quantification of ChAT activity in plasma, we determined whether ChAT activity changes in response to inflammatory challenges.

Results

Active ChAT circ

SUBMITTER: Gabalski AH 

PROVIDER: S-EPMC10922394 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature