Protein Phosphatase 2A Activation Promotes Heart Transplant Acceptance in Mice.

ABSTRACT:

Background

Although heart transplantation is the definitive treatment for heart failure in eligible patients, both acute and chronic transplant rejection frequently occur. Protein phosphatase 2A (PP2A) activity is critical in maintaining tissue and organ homeostasis. In this study, we evaluated the effect of a novel class of small molecule activators of PP2A (SMAPs) on allograft rejection in a mouse heterotopic heart transplantation model.

Methods

Recipient mice were administered with DT-061 (a pharmaceutically optimized SMAP) or vehicle by oral gavage beginning 1 d after transplantation. Histological and immunofluorescence analyses were performed to examine allograft rejection. Regulatory T cells (Treg) from recipient spleens were subjected to flow cytometry and RNA sequenc

SUBMITTER: Zhou X 

PROVIDER: S-EPMC10922415 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature