Immunosuppressive CD29⁺ Treg accumulation in the liver in mice on checkpoint inhibitor therapy.

ABSTRACT:

Objective

Liver metastases are often resistant to immune checkpoint inhibitor therapy (ICI) and portend a worse prognosis compared with metastases to other locations. Regulatory T cells (Tregs) are one of several immunosuppressive cells implicated in ICI resistance of liver tumours, but the role played by Tregs residing within the liver surrounding a tumour is unknown.

Design

Flow cytometry and single-cell RNA sequencing were used to characterise hepatic Tregs before and after ICI therapy.

Results

We found that the murine liver houses a Treg population that, unlike those found in other organs, is both highly proliferative and apoptotic at baseline. On administration of αPD-1, αPD-L1 or αCTLA4, the liver Treg population doubled regardless of the presence of an intrahep

SUBMITTER: Green BL 

PROVIDER: S-EPMC10922517 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature