Enhanced TROSY Effect in [2-¹⁹ F, 2-¹³ C] Adenosine and ATP Analogs Facilitates NMR Spectroscopy of Very Large Biological RNAs in Solution.

ABSTRACT: Large RNAs are central to cellular functions, but characterizing such RNAs remains challenging by solution NMR. We present two labeling technologies based on [2-¹⁹ F, 2-¹³ C]-adenosine, which allow the incorporation of aromatic ¹⁹ F-¹³ C spin pairs. The labels when coupled with the transverse relaxation optimized spectroscopy (TROSY) enable us to probe RNAs comprising up to 124 nucleotides. With our new [2-¹⁹ F, 2-¹³ C]-adenosine-phosphoramidite, all resonances of the human hepatitis B virus epsilon RNA could be readily assigned. With [2-¹⁹ F, 2-¹³ C]-adenosine triphosphate, the 124 nt pre-miR-17-NPSL1-RNA was produced via in vitro transcription and the TROSY spectrum of this 40 kDa [2-¹⁹ F,

SUBMITTER: Juen F 

PROVIDER: S-EPMC10922520 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature