Multiscale elasticity mapping of biological samples in 3D at optical resolution.

ABSTRACT: The mechanical properties of biological tissues have emerged as an integral determinant of tissue function in health and disease. Nonetheless, characterizing the elasticity of biological samples in 3D and at high resolution remains challenging. Here, we present a µElastography platform: a scalable elastography system that maps the elastic properties of tissues from cellular to organ scales. The platform leverages the use of a biocompatible, thermo-responsive hydrogel to deliver compressive stress to a biological sample and track its resulting deformation. By surrounding the specimen with a reference hydrogel of known Young's modulus, we are able to map the absolute values of elastic properties in biological samples. We validate the experimental and computational components of the platform

SUBMITTER: Regan K 

PROVIDER: S-EPMC10922809 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature