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Direct detection of canine picornavirus complete coding sequence in wastewater using long-range reverse-transcriptase polymerase chain reaction and long-read sequencing.

ABSTRACT: We describe four complete coding sequence (cCDS) of canine picornavirus from wastewater in Arizona, USA detected by coupling cCDS single-contig (∼7.5 kb) reverse-transcriptase polymerase chain reaction (RT-PCR) and low-cost long-read high-throughput sequencing. For viruses of medical/veterinary importance, this workflow expands possibilities of wastewater based genomic epidemiology for exploring virus evolutionary dynamics especially in low-resource settings.

SUBMITTER: Faleye TOC

PROVIDER: S-EPMC10923025 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Direct detection of canine picornavirus complete coding sequence in wastewater using long-range reverse-transcriptase polymerase chain reaction and long-read sequencing.

Faleye Temitope O C TOC Driver Erin M EM Wright Jillian M JM Halden Rolf U RU Varsani Arvind A Scotch Matthew M

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 20240108

We describe four complete coding sequence (cCDS) of canine picornavirus from wastewater in Arizona, USA detected by coupling cCDS single-contig (∼7.5 kb) reverse-transcriptase polymerase chain reaction (RT-PCR) and low-cost long-read high-throughput sequencing. For viruses of medical/veterinary importance, this workflow expands possibilities of wastewater based genomic epidemiology for exploring virus evolutionary dynamics especially in low-resource settings. ...[more]

PMID: 38199505

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