Early transcriptional changes of heavy metal resistance and multiple efflux genes in Xanthomonas campestris pv. campestris under copper and heavy metal ion stress.

ABSTRACT:

Background

Copper-induced gene expression in Xanthomonas campestris pv. campestris (Xcc) is typically evaluated using targeted approaches involving qPCR. The global response to copper stress in Xcc and resistance to metal induced damage is not well understood. However, homologs of heavy metal efflux genes from the related Stenotrophomonas genus are found in Xanthomonas which suggests that metal related efflux may also be present.

Methods and results

Gene expression in Xcc strain BrA1 exposed to 0.8 mM CuSO₄.5H₂O for 15 minutes was captured using RNA-seq analysis. Changes in expression was noted for genes related to general stress responses and oxidoreductases, biofilm formation, protein folding chaperones, heat-shock proteins, membrane lipid profile, mu

SUBMITTER: Ramnarine SDB 

PROVIDER: S-EPMC10924375 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature