Tissue-engineered and autologous pericardium in congenital heart surgery: comparative histopathological study of human vascular explants.

ABSTRACT:

Objectives

The goal of this histological study was to assess the biocompatibility of vascular patches used in the repair of congenital heart defects.

Methods

We examined tissue-engineered bovine (n = 7) and equine (n = 7) patches and autologous human pericardium (n = 7), all explanted due to functional issues or follow-up procedures. Techniques like Movat-Verhoeff, von Kossa and immunohistochemical staining were used to analyse tissue composition, detect calcifications and identify immune cells. A semi-quantitative scoring system was implemented to evaluate the biocompatibility aspects, thrombus formation, extent of pannus, inflammation of pannus, cellular response to patch material, patch degradation, calcification and neoadventitial inflammation.

Results

We observed

SUBMITTER: Eildermann K 

PROVIDER: S-EPMC10924714 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature