Kinetic properties of glucose 6-phosphate dehydrogenase and inhibition effects of several metal ions on enzymatic activity in vitro and cells.

ABSTRACT: Due to the non-degradable and persistent nature of metal ions in the environment, they are released into water bodies, where they accumulate in fish. In order to assess pollution in fish, the enzyme, glucose 6-phosphate dehydrogenase (G6PD), has been employed as a biomarker due to sensitivity to various ions. This study investigates the kinetic properties of the G6PD enzyme in yellow catfish (Pelteobagrus fulvidraco), and analyzes the effects of these metal ions on the G6PD enzyme activity in the ovarian cell line (CCO) of channel catfish (Ictalurus punctatus). IC₅₀ values and inhibition types of G6PD were determined in the metal ions Cu²⁺, Al³⁺, Zn²⁺, and Cd²⁺. While, the inhibition types of Cu²⁺ and Al³⁺ were th

SUBMITTER: Sun L 

PROVIDER: S-EPMC10924972 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature