Single-cell transcriptome reveals a novel mechanism of C-Kit⁺-liver sinusoidal endothelial cells in NASH.

ABSTRACT:

Aim

To understand how liver sinusoidal endothelial cells (LSECs) respond to nonalcoholic steatohepatitis (NASH).

Methods

We profiled single-LSEC from livers of control and MCD-fed mice. The functions of C-Kit⁺-LSECs were determined using coculture and bone marrow transplantation (BMT) methods.

Results

Three special clusters of single-LSEC were differentiated. C-Kit⁺-LSECs of cluster 0, Msr1⁺-LSECs of cluster 1 and Bmp4⁺Selp⁺-VECs of cluster 2 were revealed, and these cells with diverse ectopic expressions of genes participated in regulation of endothelial, fibrosis and lipid metabolism in NASH. The number of C-Kit⁺-primary LSECs isolated from MCD mice was lower than control mice. Immunofluorescence co-s

SUBMITTER: Li HY 

PROVIDER: S-EPMC10925010 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature