Proteomic analysis of mitochondria associated membranes in renal ischemic reperfusion injury.

ABSTRACT:

Background

The mitochondria and endoplasmic reticulum (ER) communicate via contact sites known as mitochondria associated membranes (MAMs). Many important cellular functions such as bioenergetics, mitophagy, apoptosis, and calcium signaling are regulated by MAMs, which are thought to be closely related to ischemic reperfusion injury (IRI). However, there exists a gap in systematic proteomic research addressing the relationship between these cellular processes.

Methods

A 4D label free mass spectrometry-based proteomic analysis of mitochondria associated membranes (MAMs) from the human renal proximal tubular epithelial cell line (HK-2 cells) was conducted under both normal (N) and hypoxia/reperfusion (HR) conditions. Subsequent differential proteins analysis aimed to character

SUBMITTER: Li Y 

PROVIDER: S-EPMC10925013 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature