Distinct <i>Escherichia coli</i> transcriptional profiles in the guts of recurrent UTI sufferers revealed by pangenome hybrid selection.

ABSTRACT: Low-abundance members of microbial communities are difficult to study in their native habitats. This includes Escherichia coli, a minor, but common inhabitant of the gastrointestinal tract and opportunistic pathogen, including of the urinary tract, where it is the primary pathogen. While multi-omic analyses have detailed critical interactions between uropathogenic Escherichia coli (UPEC) and the bladder that mediate UTI outcome, comparatively little is known about UPEC in its pre-infection reservoir, partly due to its low abundance there (<1% relative abundance). To accurately and sensitively explore the genomes and transcriptomes of diverse E. coli in gastrointestinal communities, we developed E. coli PanSelect which uses a set of probes designed to specificall

SUBMITTER: Young MG 

PROVIDER: S-EPMC10925322 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature