Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast.

ABSTRACT: Eukaryotic chromosome segregation requires kinetochores, multi-megadalton protein machines that assemble on the centromeres of chromosomes and mediate attachments to dynamic spindle microtubules. Kinetochores are built from numerous complexes, and understanding how they are arranged is key to understanding how kinetochores perform their multiple functions. However, an integrated understanding of kinetochore architecture has not yet been established. To address this, we purified functional, native kinetochores from Kluyveromyces marxianus and examined them by electron microscopy, cryo-electron tomography and atomic force microscopy. The kinetochores are extremely large, flexible assemblies that exhibit features consistent with prior models. We assigned kinetochore polarity by visuali

SUBMITTER: Barrero DJ 

PROVIDER: S-EPMC10925344 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature