The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies.

ABSTRACT: Viral metagenomics has fuelled a rapid change in our understanding of global viral diversity and ecology. Long-read sequencing and hybrid assembly approaches that combine long- and short-read technologies are now being widely implemented in bacterial genomics and metagenomics. However, the use of long-read sequencing to investigate viral communities is still in its infancy. While Nanopore and PacBio technologies have been applied to viral metagenomics, it is not known to what extent different technologies will impact the reconstruction of the viral community. Thus, we constructed a mock bacteriophage community of previously sequenced phage genomes and sequenced them using Illumina, Nanopore and PacBio sequencing technologies and tested a number of different assembly approaches. When using

SUBMITTER: Cook R 

PROVIDER: S-EPMC10926689 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature