Long access heroin self-administration significantly alters gut microbiome composition and structure.

ABSTRACT:

Introduction

It is well known that chronic opioid use disorder is associated with alterations in gastrointestinal (GI) function that include constipation, reduced motility, and increased bacterial translocation due to compromised gut barrier function. These signs of disrupted GI function can be associated with alterations in the gut microbiome. However, it is not known if long-access opioid self-administration has effects on the gut microbiome.

Methods

We used 16S rRNA gene sequencing to investigate the gut microbiome in three independent cohorts (N=40 for each) of NIH heterogeneous stock rats before onset of long-access heroin self-administration (i.e., naïve status), at the end of a 15-day period of self-administration, and after post-extinction reinstatement. Measures of

SUBMITTER: Greenberg JM 

PROVIDER: S-EPMC10927763 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature