Endophilin A2 controls touch and mechanical allodynia via kinesin-mediated Piezo2 trafficking.

ABSTRACT:

Background

Tactile and mechanical pain are crucial to our interaction with the environment, yet the underpinning molecular mechanism is still elusive. Endophilin A2 (EndoA2) is an evolutionarily conserved protein that is documented in the endocytosis pathway. However, the role of EndoA2 in the regulation of mechanical sensitivity and its underlying mechanisms are currently unclear.

Methods

Male and female C57BL/6 mice (8-12 weeks) and male cynomolgus monkeys (7-10 years old) were used in our experiments. Nerve injury-, inflammatory-, and chemotherapy-induced pathological pain models were established for this study. Behavioral tests of touch, mechanical pain, heat pain, and cold pain were performed in mice and nonhuman primates. Western blotting, immunostaining, co-immunoprec

SUBMITTER: Xie MX 

PROVIDER: S-EPMC10929226 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature