Geometry and length control of 3D engineered heart tissues using direct laser writing.

ABSTRACT: Geometry and mechanical characteristics of the environment surrounding the Engineered Heart Tissues (EHT) affect their structure and function. Here, we employed a 3D tissue culture platform fabricated using two-photon direct laser writing with a high degree of accuracy to control parameters that are relevant to EHT maturation. Using this platform, we first explore the effects of geometry based on two distinct shapes: a rectangular seeding well with two attachment sites, and a stadium-like seeding well with six attachment sites that are placed symmetrically along hemicylindrical membranes. The former geometry promotes uniaxial contraction of the tissues; the latter additionally induces diagonal fiber alignment. We systematically increase the length of the seeding wells for both configuratio

SUBMITTER: Karakan MC 

PROVIDER: S-EPMC10929702 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature