Dataset Information

Establishment and Characterization of a Primary Fibroblast Cell Culture from the Amazonian Manatee (Trichechus inunguis).

ABSTRACT: The vulnerable status of the Amazon manatee, Trichechus inunguis, indicates the need to seek measures to guarantee its conservation. In this context, the cultivation of cells in vitro is a strategy that should at least guarantee the preservation of their genetic material. Thus, we established for the first time a primary culture of Amazonian manatee fibroblasts (TINsf) from a skin biopsy of a young male. Karyotypic analysis of the 3rd, 7th, and 12th passages confirmed the taxonomic identity of the species T. inunguis (2n = 56/NF = 92) and indicated that this culture presents genomic stability. Gene and protein expression of vimentin at the 13th passage show the predominant presence of fibroblasts in TINsf. To test the cell line's responsiveness to materials and demonstrate a possible application of this culture, it was exposed to andiroba seed oil (ASO), and its viability and proliferative capacity were evaluated. ASO demonstrated toxic effects at the highest concentrations and longest exposure times tested, reproducing results observed in human cultures, indicating the applicability of TINsf in toxicological and biotechnological studies. After cryopreservation, the TINsf line maintained its proliferative potential, indicating the establishment of a new culture available for future studies.

SUBMITTER: Tavares FDS

PROVIDER: S-EPMC10931340 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Publications

Establishment and Characterization of a Primary Fibroblast Cell Culture from the Amazonian Manatee (<i>Trichechus inunguis</i>).

Tavares Flávia Dos Santos FDS Martins Cesar C Delella Flávia Karina FK Nascimento Luís Adriano Santos do LASD Rodrigues Angélica Lúcia Figueiredo ALF Moreira Sávia S Cardoso Adauto Lima AL Noronha Renata Coelho Rodrigues RCR

Animals : an open access journal from MDPI 20240222 5

The vulnerable status of the Amazon manatee, <i>Trichechus inunguis</i>, indicates the need to seek measures to guarantee its conservation. In this context, the cultivation of cells in vitro is a strategy that should at least guarantee the preservation of their genetic material. Thus, we established for the first time a primary culture of Amazonian manatee fibroblasts (TINsf) from a skin biopsy of a young male. Karyotypic analysis of the 3rd, 7th, and 12th passages confirmed the taxonomic identi ...[more]

PMID: 38473072

Similar Datasets

Project description:A retrospective study of admission data of 401 West Indian manatees (Trichechus manatus latirostris) presented to the David A. Straz Jr. Manatee Critical Care Center at ZooTampa at Lowry Park (ZooTampa) for rehabilitation from August 1991 through October 2017. Causes of admittance, location of rescue, gender, and age class were all recorded for each manatee admitted. Admittance categories as defined by the Florida Fish and Wildlife Conservation Commission (FWC) included watercraft collisions, natural causes, entanglement, entrapment, orphaned calves, captive born, mothers of rescued calves, calves of rescued mothers, human, and other. The admitted population was primarily from the southwest and northwest coasts and related waterways of Florida. The gender difference was relatively equivocal (54% female) while the adults comprised 79% of the admissions. The overall total admissions increased steadily over the study period as did the admissions for each individual categories of admission. Watercraft collisions and natural causes combined were 71% of all admissions for the entire study period and are the dominant causes of admission. Watercraft collisions are more likely to occur during May through October, whereas natural causes of admittance are more likely to occur between December and March. Rehabilitated manatees may reduce overall manatee mortality and can provide insight into population-based health concerns if evaluated appropriately. Future efforts can incorporate physical examination findings, hematology, biochemistry profiles, and ancillary diagnostic testing to continue to improve the individual welfare of this marine mammal in its natural range. Admissions data could also potentially serve the wider conservation and recovery efforts if it is proven that the data obtained is at least as informative as that obtained by the carcass salvage program. Limited conservation resources could then be re-directed as new challenges arise with the expanding population and potentially expanding range of this species.

Dataset Information

Establishment and Characterization of a Primary Fibroblast Cell Culture from the Amazonian Manatee (Trichechus inunguis).

Publications

Establishment and Characterization of a Primary Fibroblast Cell Culture from the Amazonian Manatee (<i>Trichechus inunguis</i>).

Similar Datasets

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