Dataset Information

Identification of bZIP Transcription Factors That Regulate the Development of Leaf Epidermal Cells in Arabidopsis thaliana by Single-Cell RNA Sequencing.

ABSTRACT: Epidermal cells are the main avenue for signal and material exchange between plants and the environment. Leaf epidermal cells primarily include pavement cells, guard cells, and trichome cells. The development and distribution of different epidermal cells are tightly regulated by a complex transcriptional regulatory network mediated by phytohormones, including jasmonic acid, and transcription factors. How the fate of leaf epidermal cells is determined, however, is still largely unknown due to the diversity of cell types and the complexity of their regulation. Here, we characterized the transcriptional profiles of epidermal cells in 3-day-old true leaves of Arabidopsis thaliana using single-cell RNA sequencing. We identified two genes encoding BASIC LEUCINE-ZIPPER (bZIP) transcription factors, namely bZIP25 and bZIP53, which are highly expressed in pavement cells and early-stage meristemoid cells. Densities of pavement cells and trichome cells were found to increase and decrease, respectively, in bzip25 and bzip53 mutants, compared with wild-type plants. This trend was more pronounced in the presence of jasmonic acid, suggesting that these transcription factors regulate the development of trichome cells and pavement cells in response to jasmonic acid.

SUBMITTER: Wu R

PROVIDER: S-EPMC10931525 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Identification of bZIP Transcription Factors That Regulate the Development of Leaf Epidermal Cells in <i>Arabidopsis thaliana</i> by Single-Cell RNA Sequencing.

Wu Rui R Liu Zhixin Z Sun Susu S Qin Aizhi A Liu Hao H Zhou Yaping Y Li Weiqiang W Liu Yumeng Y Hu Mengke M Yang Jincheng J Rochaix Jean-David JD An Guoyong G Herrera-Estrella Luis L Tran Lam-Son Phan LP Sun Xuwu X

International journal of molecular sciences 20240222 5

Epidermal cells are the main avenue for signal and material exchange between plants and the environment. Leaf epidermal cells primarily include pavement cells, guard cells, and trichome cells. The development and distribution of different epidermal cells are tightly regulated by a complex transcriptional regulatory network mediated by phytohormones, including jasmonic acid, and transcription factors. How the fate of leaf epidermal cells is determined, however, is still largely unknown due to the ...[more]

PMID: 38473801

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Project description:BackgroundStromules are dynamic tubular structures emerging from the surface of plastids that are filled with stroma. Despite considerable progress in understanding the importance of certain cytoskeleton elements and motor proteins for stromule maintenance, their function within the plant cell is still unknown. It has been suggested that stromules facilitate the exchange of metabolites and/or signals between plastids and other cell compartments by increasing the cytosolically exposed plastid surface area but experimental evidence for the involvement of stromules in metabolic processes is not available. The frequent occurrence of stromules in both sink tissues and heterotrophic cell cultures suggests that the presence of carbohydrates in the extracellular space is a possible trigger of stromule formation. We have examined this hypothesis with induction experiments using the upper epidermis from rosette leaves of Arabidopsis thaliana as a model system.ResultsWe found that the stromule frequency rises significantly if either sucrose or glucose is applied to the apoplast by vacuum infiltration. In contrast, neither fructose nor sorbitol or mannitol are capable of inducing stromule formation which rules out the hypothesis that stromule induction is merely the result of changes in the osmotic conditions. Stromule formation depends on translational activity in the cytosol, whereas protein synthesis within the plastids is not required. Lastly, stromule induction is not restricted to the plastids of the upper epidermis but is similarly observed also with chloroplasts of the palisade parenchyma.ConclusionsThe establishment of an experimental system allowing the reproducible induction of stromules by vacuum infiltration of leaf tissue provides a suitable tool for the systematic analysis of conditions and requirements leading to the formation of these dynamic organelle structures. The applicability of the approach is demonstrated here by analyzing the influence of apoplastic sugar solutions on stromule formation. We found that only a subset of sugars generated in the primary metabolism of plants induce stromule formation, which is furthermore dependent on cytosolic translational activity. This suggests regulation of stromule formation by sugar sensing mechanisms and a possible role of stromules in carbohydrate metabolism and metabolite exchange.

Dataset Information

Identification of bZIP Transcription Factors That Regulate the Development of Leaf Epidermal Cells in Arabidopsis thaliana by Single-Cell RNA Sequencing.

Publications

Identification of bZIP Transcription Factors That Regulate the Development of Leaf Epidermal Cells in <i>Arabidopsis thaliana</i> by Single-Cell RNA Sequencing.

Similar Datasets

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