FlashPCR: Revolutionising qPCR by Accelerating Amplification through Low ∆T Protocols.

ABSTRACT: Versatility, sensitivity, and accuracy have made the real-time polymerase chain reaction (qPCR) a crucial tool for research, as well as diagnostic applications. However, for point-of-care (PoC) use, traditional qPCR faces two main challenges: long run times mean results are not available for half an hour or more, and the requisite high-temperature denaturation requires more robust and power-demanding instrumentation. This study addresses both issues and revises primer and probe designs, modified buffers, and low ∆T protocols which, together, speed up qPCR on conventional qPCR instruments and will allow for the development of robust, point-of-care devices. Our approach, called "FlashPCR", uses a protocol involving a 15-second denaturation at 79 °C, followed by repeated cycling for 1 s at 79

SUBMITTER: Bustin SA 

PROVIDER: S-EPMC10932470 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature