Berberine alleviates concanavalin A-induced autoimmune hepatitis in mice by modulating the gut microbiota.

ABSTRACT:

Background

Autoimmune hepatitis (AIH) is an immune-mediated liver disease of unknown etiology accompanied by intestinal dysbiosis and a damaged intestinal barrier. Berberine (BBR) is a traditional antibacterial medicine that has a variety of pharmacological properties. It has been reported that BBR alleviates AIH, but relevant mechanisms remain to be fully explored.

Methods

BBR was orally administered at doses of 100 mg⋅kg-1⋅d-1 for 7 days to mice before concanavalin A-induced AIH model establishment. Histopathological, immunohistochemical, immunofluorescence, western blotting, ELISA, 16S rRNA analysis, flow cytometry, real-time quantitative PCR, and fecal microbiota transplantation studies were performed to ascertain BBR effects and mechanisms in AIH mice.

Results

We

SUBMITTER: Yang H 

PROVIDER: S-EPMC10932532 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature