Electrochemical labelling of hydroxyindoles with chemoselectivity for site-specific protein bioconjugation.

ABSTRACT: Electrochemistry has recently emerged as a powerful approach in small-molecule synthesis owing to its numerous attractive features, including precise control over the fundamental reaction parameters, mild reaction conditions and innate scalability. Even though these advantages also make it an attractive strategy for chemoselective modification of complex biomolecules such as proteins, such applications remain poorly developed. Here we report an electrochemically promoted coupling reaction between 5-hydroxytryptophan (5HTP) and simple aromatic amines-electrochemical labelling of hydroxyindoles with chemoselectivity (eCLIC)-that enables site-specific labelling of full-length proteins under mild conditions. Using genetic code expansion technology, the 5HTP residue can be incorporated into pre

SUBMITTER: Loynd C 

PROVIDER: S-EPMC10932882 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature