Transcriptome-wide 1-methyladenosine functional profiling of messenger RNA and long non-coding RNA in bladder cancer.

ABSTRACT: Introduction: Post-transcriptional RNA modifications are crucial regulators of tumor development and progression. In many biological processes, N¹-methyladenosine (m¹A) plays a key role. However, little is known about the links between chemical modifications of messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) and their function in bladder cancer (BLCA). Methods: Methylated RNA immunoprecipitation sequencing and RNA sequencing were performed to profile mRNA and lncRNA m¹A methylation and expression in BLCA cells, with or without stable knockdown of the m¹A methyltransferase tRNA methyltransferase 61A (TRMT61A). Results: The analysis of differentially methylated gene sites identified 16,941 peaks, 6,698 mRNAs, and 10,24

SUBMITTER: Yin JJ 

PROVIDER: S-EPMC10933092 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature