Defining a metagenomic threshold for detecting low abundances of Providencia alcalifaciens in canine faecal samples.

ABSTRACT:

Introduction

Acute haemorrhagic diarrhoea syndrome (AHDS) in dogs is a condition of unknown aetiology. Providencia alcalifaciens is suspected to play a role in the disease as it was commonly found in dogs suffering from AHDS during a Norwegian outbreak in 2019. The role of this bacterium as a constituent of the canine gut microbiota is unknown, hence this study set out to investigate its occurrence in healthy dogs using metagenomics.

Materials and methods

To decrease the likelihood of false detection, we established a metagenomic threshold for P. alcalifaciens by spiking culture-negative stool samples with a range of bacterial dilutions and analysing these by qPCR and shotgun metagenomics. The detection limit for P. alcalifaciens was determined and used

SUBMITTER: Aardal AM 

PROVIDER: S-EPMC10933104 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature