Unraveling the molecular interactions between α7 nicotinic receptor and a RIC3 variant associated with backward speech.

ABSTRACT: Recent work putatively linked a rare genetic variant of the chaperone Resistant to Inhibitors of acetylcholinesterase (RIC3) (NM_024557.4:c.262G > A, NP_078833.3:p.G88R) to a unique ability to speak backwards, a language skill that is associated with exceptional working memory capacity. RIC3 is important for the folding, maturation, and functional expression of α7 nicotinic acetylcholine receptors (nAChR). We compared and contrasted the effects of RIC3G88R on assembly, cell surface expression, and function of human α7 receptors using fluorescent protein tagged α7 nAChR and Förster resonance energy transfer (FRET) microscopy imaging in combination with functional assays and ¹²⁵I-α-bungarotoxin binding. As expected, the wild-type RIC3 protein was found to increase both cell surfac

SUBMITTER: Pradhan A 

PROVIDER: S-EPMC10933150 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature