Crosstalk between purinergic receptor P2Y₁₁ and chemokine receptor CXCR7 is regulated by CXCR4 in human macrophages.

ABSTRACT: P2Y₁₁ is a G protein-coupled ATP receptor that activates IL-1 receptor (IL-1R) in a cyclic AMP dependent manner. In human macrophages, P2Y₁₁/IL-1R crosstalk with CCL20 as a prime target is controlled by phosphodiesterase 4 (PDE4), which mediates breakdown of cyclic AMP. Here, we used gene expression analysis to identify activation of CXCR4 and CXCR7 as a hallmark of P2Y₁₁ signaling. We found that PDE4 inhibition with rolipram boosts P2Y₁₁/IL-1R-induced upregulation of CXCR7 expression and CCL20 production in an epidermal growth factor receptor dependent manner. Using an astrocytoma cell line, naturally expressing CXCR7 but lacking CXCR4, P2Y₁₁/IL-1R activation effectively induced and CXCR7 agonist TC14012 enhanced CCL20 production even

SUBMITTER: Klaver D 

PROVIDER: S-EPMC10933201 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature