Single-cell division tracing and transcriptomics reveal cell types and differentiation paths in the regenerating lung.

ABSTRACT: Understanding the molecular and cellular processes involved in lung epithelial regeneration may fuel the development of therapeutic approaches for lung diseases. We combine mouse models allowing diphtheria toxin-mediated damage of specific epithelial cell types and parallel GFP-labeling of functionally dividing cells with single-cell transcriptomics to characterize the regeneration of the distal lung. We uncover cell types, including Krt13⁺ basal and Krt15⁺ club cells, detect an intermediate cell state between basal and goblet cells, reveal goblet cells as actively dividing progenitor cells, and provide evidence that adventitial fibroblasts act as supporting cells in epithelial regeneration. We also show that diphtheria toxin-expressing cells can persist in the lung,

SUBMITTER: Martins LR 

PROVIDER: S-EPMC10933319 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature