Filamentous-Actin-Mimicking Nanoplatform for Enhanced Cytosolic Protein Delivery.

ABSTRACT: Despite the potential of protein therapeutics, the cytosolic delivery of proteins with high efficiency and bioactivity remains a significant challenge owing to exocytosis and lysosomal degradation after endocytosis. Therefore, it is important to develop a safe and efficient strategy to bypass endocytosis. Inspired by the extraordinary capability of filamentous-actin (F-actin) to promote cell membrane fusion, a cyanine dye assembly-containing nanoplatform mimicking the structure of natural F-actin is developed. The nanoplatform exhibits fast membrane fusion to cell membrane mimics and thus enters live cells through membrane fusion and bypasses endocytosis. Moreover, it is found to efficiently deliver protein cargos into live cells and quickly release them into the cytosol, leading to high p

SUBMITTER: Xia Y 

PROVIDER: S-EPMC10933650 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature