Computational Analysis of the Tripartite Interaction of Phasins (PhaP4 and 5)-Sigma Factor (σ²⁴)-DNA of Azospirillum brasilense Sp7.

ABSTRACT: Azospirillum brasilense Sp7 produces PHB, which is covered by granule-associated proteins (GAPs). Phasins are the main GAPs. Previous studies have shown phasins can regulate PHB synthesis. When A. brasilense grows under stress conditions, it uses sigma factors to transcribe genes for survival. One of these factors is the σ²⁴ factor. This study determined the possible interaction between phasins and the σ²⁴ factor or phasin-σ²⁴ factor complex and DNA. Three-dimensional structures of phasins and σ²⁴ factor structures were predicted using the I-TASSER and SWISS-Model servers, respectively. Subsequently, a molecular docking between phasins and the σ²⁴ factor was performed using the ClusPro 2.0 server, followed by molecular do

SUBMITTER: Aguilar-Carrillo Y 

PROVIDER: S-EPMC10934627 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature