Establishing an ANO1-Based Cell Model for High-Throughput Screening Targeting TRPV4 Regulators.

ABSTRACT: Transient receptor potential vanilloid 4 (TRPV4) is a widely expressed cation channel that plays an important role in many physiological and pathological processes. However, most TRPV4 drugs carry a risk of side effects. Moreover, existing screening methods are not suitable for the high-throughput screening (HTS) of drugs. In this study, a cell model and HTS method for targeting TRPV4 channel drugs were established based on a calcium-activated chloride channel protein 1 Anoctamin 1 (ANO1) and a double mutant (YFP-H148Q/I152L) of the yellow fluorescent protein (YFP). Patch-clamp experiments and fluorescence quenching kinetic experiments were used to verify that the model could sensitively detect changes in intracellular Ca²⁺ concentration. The functionality of the TRPV4 cell mode

SUBMITTER: Zheng K 

PROVIDER: S-EPMC10934975 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature