Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu²⁺ in Cells.

ABSTRACT: Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity recognition for Cu²⁺. With the combination of Cu²⁺, the probe was subjected to fluorescence quenching. The research suggested that the probe FP carried out the highly sensitive detection of Cu²⁺ with detection limits of 1.7 nM. The fluorescence quenching of fluorescamine was induced by Cu²⁺ perhaps due to the PET (photoinduced electron transfer) mechanism. The FP-Cu²⁺ complex shows weak fluorescence, which is likely due to the PET quenching

SUBMITTER: Ren J 

PROVIDER: S-EPMC10935257 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature