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Precisely modulating the chromatin tracker via substituent engineering: reporting pathological oxidative stress during mitosis.

ABSTRACT: An in-depth understanding of cancer-cell mitosis presents unprecedented advantages for solving metastasis and proliferation of tumors, which has aroused great interest in visualizing the behavior via a luminescence tool. We developed a fluorescent molecule CBTZ-yne based on substituent engineering to acquire befitting lipophilicity and electrophilicity for anchoring lipid droplets and the nucleus, in which the low polarity environment and nucleic acids triggered a "weak-strong" fluorescence and "short-long" fluorescence-lifetime response. Meaningfully, CBTZ-yne visualized chromatin condensation, alignment, pull-push, and separation as well as lipid droplet dynamics, for the first time, precisely unveiling the asynchronous cellular mitosis processes affected by photo-generation reactive oxygen species according to the subtle change of fluorescence-lifetime. Our work suggested a new guideline for tracking the issue of the proliferation of malignant tumors in photodynamic therapy.

SUBMITTER: Li J 

PROVIDER: S-EPMC10935666 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Precisely modulating the chromatin tracker <i>via</i> substituent engineering: reporting pathological oxidative stress during mitosis.

Li Jinsong J   Ni Yingyong Y   Wang Junjun J   Zhu Yicai Y   Wang Aidong A   Zhu Xiaojiao X   Sun Xianshun X   Wang Sen S   Li Dandan D   Zhou Hongping H  

Chemical science 20240131 11

An in-depth understanding of cancer-cell mitosis presents unprecedented advantages for solving metastasis and proliferation of tumors, which has aroused great interest in visualizing the behavior <i>via</i> a luminescence tool. We developed a fluorescent molecule CBTZ-yne based on substituent engineering to acquire befitting lipophilicity and electrophilicity for anchoring lipid droplets and the nucleus, in which the low polarity environment and nucleic acids triggered a "weak-strong" fluorescen  ...[more]