Antidepressant fluoxetine alleviates colitis by reshaping intestinal microenvironment.

ABSTRACT:

Background

The impact of antidepressants on Inflammatory bowel diseases (IBD) has been extensively studied. However, the biological effects and molecular mechanisms of antidepressants in alleviating colitis remain unclear.

Methods

We systematically assessed how antidepressants (fluoxetine, fluvoxamine and venlafaxine) affected IBD and chose fluoxetine, the most effective one, for mechanism studies. We treated the C56BL/6 mice of the IBD model with fluoxetine and their controls. We initially assessed the severity of intestinal inflammation in mice by body weight loss, disease Activity Index scores and the length of the colon. The H&E staining and immunohistochemical staining of MUC2 of colon sections were performed to observe the pathological changes. RT-qPCR and western blot

SUBMITTER: Teng S 

PROVIDER: S-EPMC10935910 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature