ClusterAlign: A fiducial tracking and tilt series alignment tool for thick sample tomography.

ABSTRACT: Thick specimens, as encountered in cryo-scanning transmission electron tomography, offer special challenges to conventional reconstruction workflows. The visibility of features, including gold nanoparticles introduced as fiducial markers, varies strongly through the tilt series. As a result, tedious manual refinement may be required in order to produce a successful alignment. Information from highly tilted views must often be excluded to the detriment of axial resolution in the reconstruction. We introduce here an approach to tilt series alignment based on identification of fiducial particle clusters that transform coherently in rotation, essentially those that lie at similar depth. Clusters are identified by comparison of tilted views with a single untilted reference, rather than with adj

SUBMITTER: Seifer S 

PROVIDER: S-EPMC10936405 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature