Ot2Rec: A semi-automatic, extensible, multi-software tomographic reconstruction workflow.

ABSTRACT: Electron cryo-tomography is an imaging technique for probing 3D structures with at the nanometer scale. This technique has been used extensively in the biomedical field to study the complex structures of proteins and other macromolecules. With the advancement in technology, microscopes are currently capable of producing images amounting to terabytes of data per day, posing great challenges for scientists as the speed of processing of the images cannot keep up with the ever-higher throughput of the microscopes. Therefore, automation is an essential and natural pathway on which image processing-from individual micrographs to full tomograms-is developing. In this paper, we present Ot2Rec, an open-source pipelining tool which aims to enable scientists to build their own processing workflows in

SUBMITTER: Yee NB 

PROVIDER: S-EPMC10936412 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature