Subcellular Imaging of Neuronal Calcium Handling In Vivo.

ABSTRACT: Calcium (Ca²⁺) imaging has been largely used to examine neuronal activity, but it is becoming increasingly clear that subcellular Ca²⁺ handling is a crucial component of intracellular signaling. The visualization of subcellular Ca²⁺ dynamics in vivo, where neurons can be studied in their native, intact circuitry, has proven technically challenging in complex nervous systems. The transparency and relatively simple nervous system of the nematode Caenorhabditis elegans enable the cell-specific expression and in vivo visualization of fluorescent tags and indicators. Among these are fluorescent indicators that have been modified for use in the cytoplasm as well as various subcellular compartments, such as the mitochondria. This protocol enables non-ratiometric C

SUBMITTER: Doser R 

PROVIDER: S-EPMC10937071 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature