In vitro reconstitution of chromatin domains shows a role for nucleosome positioning in 3D genome organization.

ABSTRACT: Eukaryotic genomes are organized into chromatin domains. The molecular mechanisms driving the formation of these domains are difficult to dissect in vivo and remain poorly understood. Here we reconstitute Saccharomyces cerevisiae chromatin in vitro and determine its 3D organization at subnucleosome resolution by micrococcal nuclease-based chromosome conformation capture and molecular dynamics simulations. We show that regularly spaced and phased nucleosome arrays form chromatin domains in vitro that resemble domains in vivo. This demonstrates that neither loop extrusion nor transcription is required for basic domain formation in yeast. In addition, we find that the boundaries of reconstituted domains correspond to nucleosome-free regions and that insulation strength scales with their width

SUBMITTER: Oberbeckmann E 

PROVIDER: S-EPMC10937381 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature