Obesity-induced downregulation of miR-192 exacerbates lipopolysaccharide-induced acute lung injury by promoting macrophage activation.

ABSTRACT:

Background

Macrophage activation may play a crucial role in the increased susceptibility of obese individuals to acute lung injury (ALI). Dysregulation of miRNA, which is involved in various inflammatory diseases, is often observed in obesity. This study aimed to investigate the role of miR-192 in lipopolysaccharide (LPS)-induced ALI in obese mice and its mechanism of dysregulation in obesity.

Methods

Human lung tissues were obtained from obese patients (BMI ≥ 30.0 kg/m²) and control patients (BMI 18.5-24.9 kg/m²). An obese mouse model was established by feeding a high-fat diet (HFD), followed by intratracheal instillation of LPS to induce ALI. Pulmonary macrophages of obese mice were depleted through intratracheal instillation of clodronate liposomes.

SUBMITTER: Wu S 

PROVIDER: S-EPMC10938800 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature